Journal
MICROORGANISMS
Volume 8, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/microorganisms8101551
Keywords
biofilm; MRSA; silver ion; silver sulfadiazine; wound infections
Categories
Funding
- KAKENHI [17K10038]
- ALCARE Co., Ltd., Tokyo Japan
- Grant of The Clinical Research Promotion Foundation (2019)
- Fukuoka University Research Grand for Science Progression
- Grants-in-Aid for Scientific Research [17K10038] Funding Source: KAKEN
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Methicillin-resistant Staphylococcus aureus (MRSA), the most commonly detected drug-resistant microbe in hospitals, adheres to substrates and forms biofilms that are resistant to immunological responses and antimicrobial drugs. Currently, there is a need to develop alternative approaches for treating infections caused by biofilms to prevent delays in wound healing. Silver has long been used as a disinfectant, which is non-specific and has relatively low cytotoxicity. Silver sulfadiazine (SSD) is a chemical complex clinically used for the prevention of wound infections after injury. However, its effects on biofilms are still unclear. In this study, we aimed to analyze the mechanisms underlying SSD action on biofilms formed by MRSA. The antibacterial effects of SSD were a result of silver ions and not sulfadiazine. Ionized silver from SSD in culture media was lower than that from silver nitrate; however, SSD, rather than silver nitrate, eradicated mature biofilms by bacterial killing. In SSD, sulfadiazine selectively bound to biofilms, and silver ions were then liberated. Consequently, the addition of an ion-chelator reduced the bactericidal effects of SSD on biofilms. These results indicate that SSD is an effective compound for the eradication of biofilms; thus, SSD should be used for the removal of biofilms formed on wounds.
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