Lactobacillus paracaseiKBL382 administration attenuates atopic dermatitis by modulating immune response and gut microbiota

Administration of probiotics has been linked to immune regulation and changes in gut microbiota composition, with effects on atopic dermatitis (AD). In this study, we investigated amelioration of the symptoms of AD usingLactobacillus paracaseiKBL382 isolated from the feces of healthy Koreans. Mice withDermatophagoides farinaeextract (DFE)-induced AD were fed 1 x 10(9)CFU d(-1)ofL. paracaseiKBL382 for 4 weeks. Oral administration ofL. paracaseiKBL382 significantly reduced AD-associated skin lesions, epidermal thickening, serum levels of immunoglobulin E, and immune cell infiltration.L. paracaseiKBL382-treated mice showed decreased production of T helper (Th)1-, Th2-, and Th17-type cytokines, including thymic stromal lymphopoietin, thymus, and activation-regulated chemokine, and macrophage-derived chemokine, and increased production of the anti-inflammatory cytokine IL-10 and transforming growth factor-beta in skin tissue. Intake ofL. paracaseiKBL382 also increased the proportion of CD4+ CD25+ Foxp3+ regulatory T cells in mesenteric lymph nodes. In addition, administration ofL. paracaseiKBL382 dramatically changed the composition of gut microbiota in AD mice. Administration of KBL382 significantly ameliorates AD-like symptoms by regulating the immune response and altering the composition of gut microbiota.