Bisphenol A decreases progesterone synthesis in human ovarian granulosa cells

Evidences indicate that the decline of female fertility is becoming a common problem over the past few decades. Environmental exposure of Bisphenol A (BPA) has been considered as an endocrine-disrupting chemical deleteriously affecting human reproductions. To better understand the effect of BPA exposure on human ovarian granulosa cells, we treated human ovarian granulosa cell line (KGN) with increasing concentrations (0.1, 1, 10, and 100 mu M) of BPA for 24 hr. About 0.1, 1, and 10 mu M BPA did not significantly affect the viability of KGN while 100 mu M of BPA caused a statistically significant decrease in the viability of KGN. Treatment KGN with 10 mu M BPA resulted in a significant decrease in progesterone biosynthesis. The treatment also significantly increased the expression of ATP binding cassette subfamily A member 1 (ABCA1) and steroidogenic acute regulatory protein (STAR). In the current study, exposure to BPA could lead to decreased progesterone production probably through the upregulation of ABCA1 in human granulosa cells.