A versatile biosensing platform coupling CRISPR-Cas12a and aptamers for detection of diverse analytes

Rapid and sensitive detection of various analytes is in high demand. Apart from its application in genome editing, CRISPR-Cas also shows promises in nucleic acid detection applications. To further exploit the potential of CRISPR-Cas for detection of diverse analytes, we present a versatile biosensing platform that couples the excellent affinity of aptamers for broad-range analytes with the collateral single-strand DNA cleavage activity of CRISPR-Cas12a. We demonstrated that the biosensors developed by this platform can be used to detect protein and small molecule in human serum with a complicated background, i.e., the tumor marker alpha fetoprotein and cocaine with the detection limits of 0.07 fmol/L and 0.34 mu mol/L, respectively, highlighting the advantages of simplicity, sensitivity, short detection time, and low cost compared with the state-of-the-art biosensing approaches. Altogether, this biosensing platform with plug-and-play design show great potential in the detection of diverse analytes. (C) 2020 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved.

Automated summary

This study introduces a versatile biosensing platform that combines CRISPR-Cas with aptamers for detecting proteins and small molecules in human serum. The platform offers advantages of simplicity, sensitivity, short detection time, and low cost, showing great potential in diverse analyte detection.