Palladium nanoplates scotch breast cancer lung metastasis by constraining epithelial-mesenchymal transition

Metastasis accounts for the majority of cancer deaths in many tumor types including breast cancer. Epithelial-mesenchymal transition (EMT) is the driving force for the occurrence and progression of metastasis, however, no targeted strategies to block the EMT program are currently available to combat metastasis. Diverse engineered nanomaterials (ENMs) have been reported to exert promising anti-cancer effects, however, no ENMs have been designed to target EMT. Palladium (Pd) nanomaterials, a type of ENM, have received substantial attention in nanomedicine due to their favorable photothermal performance for cancer therapeutics. Herein, Pd nanoplates (PdPL) were found to be preferentially biodistributed to both primary tumors and metastatic tumors. Importantly, PdPL showed a significant inhibition of lung metastasis with and without near-infrared (NIR) irradiation. Mechanistic investigations revealed that EMT was significantly compromised in breast cancer cells upon the PdPL treatment, which was partially due to the inhibition of the transforming growth factor-beta (TGF-beta) signaling. Strikingly, the PdPL was found to directly interact with TGF-beta proteins to diminish TGF-beta functions in activating its downstream signaling, as evidenced by the reduced phosphorylation of Smad2. Notably, TGF-beta-independent pathways were also involved in undermining EMT and other important biological processes that are necessary for metastasis. Additionally, NIR irradiation elicited synergistic effects on PdPL-induced inhibition of primary tumors and metastasis. In summary, these results revealed that the PdPL remarkably curbed metastasis by inhibiting EMT signaling, thereby indicating the promising potential of PdPL as a therapeutic agent for treating breast cancer metastasis.

Automated summary

Palladium nanoplates inhibit lung metastasis by suppressing EMT signaling in breast cancer cells and interacting with TGF-beta proteins, leading to reduced downstream signaling activation. NIR irradiation enhances the inhibitory effects of PdPL on both primary tumors and metastasis.