Journal
CURRENT OPINION IN STRUCTURAL BIOLOGY
Volume 38, Issue -, Pages 129-136Publisher
CURRENT BIOLOGY LTD
DOI: 10.1016/j.sbi.2016.06.005
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Funding
- Swiss Innovation Promotion Agency [10203.1PFLS-LS]
- EPFL
- Swiss Vaccine Research Institute [9733_531180]
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We highlight recent developments for the production of recombinant proteins from suspension-adapted mammalian cell lines. We discuss the generation of stable cell lines using transposons and lentivirus vectors (non-targeted transgene integration) and site-specific recombinases (targeted transgene integration). Each of these methods results in the generation of cell lines with protein yields that are generally superior to those achievable through classical plasmid transfection that depends on the integration of the transfected DNA by non-homologous DNA end-joining. This is the main reason why these techniques can also be used for the generation of stable cell pools, heterogenous populations of recombinant cells generated by gene delivery and genetic selection without resorting to single cell cloning. This allows the time line from gene transfer to protein production to be reduced.
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