Journal
AMB EXPRESS
Volume 10, Issue 1, Pages -Publisher
SPRINGER
DOI: 10.1186/s13568-020-01083-7
Keywords
Ammonia; Metabolic profiling; Metabolic engineering; Food by-products; Biorefinery
Categories
Funding
- Kikkoman Corporation
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Ammonia is used as a fertilizer for agriculture, chemical raw material, and carrier for transporting hydrogen, and with economic development, the demand for ammonia has increased. The Haber-Bosch process, which is the main method for producing ammonia, can produce ammonia with high efficiency. However, since it consumes a large amount of fossil energy, it is necessary to develop an alternative method for producing ammonia with less environmental impact. Ammonia production from food by-products is an appealing production process owing to unused resource usage, including waste, and mild reaction conditions. However, when food by-products and biomass are used as feedstocks, impurities often reduce productivity. Using metabolic profiling, glucose was identified as a potential inhibitor of ammonia production from impure food by-products. We constructed the recombinantEscherichia coli, in which glucose uptake was reduced byptsGgene disruption and amino acid catabolism was promoted byglnAgene disruption. Ammonia production efficiency from okara, a food by-product, was improved in this strain; 35.4 mM ammonia was produced (47% yield). This study might provide a strategy for efficient ammonia production from food by-products.
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