Journal
ADVANCED SCIENCE
Volume 7, Issue 23, Pages -Publisher
WILEY
DOI: 10.1002/advs.202001854
Keywords
cell cloning; fluid walls; immiscible jet; microfluidics; Poisson distribution
Categories
Funding
- iotaSciences Ltd
- William H.G. FitzGerald Scholarship
- Royal Society University Research Fellowship
- Impact Acceleration Account of the Biotechnology and Biological Sciences Research Council
- Medical Research Council [MC_PC_15029]
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There is an unmet demand for microfluidics in biomedicine. This paper describes contactless fabrication of microfluidic circuits on standard Petri dishes using just a dispensing needle, syringe pump, three-way traverse, cell-culture media, and an immiscible fluorocarbon (FC40). A submerged microjet of FC40 is projected through FC40 and media onto the bottom of a dish, where it washes media away to leave liquid fluorocarbon walls pinned to the substrate by interfacial forces. Such fluid walls can be built into almost any imaginable 2D circuit in minutes, which is exploited to clone cells in a way that beats the Poisson limit, subculture adherent cells, and feed arrays of cells continuously for a week. This general method should have wide application in biomedicine.
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