Journal
STEM CELL REPORTS
Volume 15, Issue 3, Pages 749-760Publisher
CELL PRESS
DOI: 10.1016/j.stemcr.2020.07.020
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Funding
- National Key Research and Development Program of China, China [2018YFA0800200, 2018YFA0801000, 2016YFA0100500]
- Strategic Priority Research Program of the Chinese Academy of Sciences, China [XDA16010207]
- National Natural Science Foundation of China, China [31830061, 31425016, 81530004]
- Youth Innovation Promotion Association, CAS, China [2016083]
- National Cancer Center grant, Korea [NCC1310860]
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Cell transplantation into immunodeficient recipients is a widely used approach to study stem cell and cancer biology; however, studying cell states post transplantation in vivo is inconvenient in mammals. Here, we generated a foxn1/Casper mutant zebrafish that is transparent and exhibits T cell deficiency. By employing the line for hematopoietic stem cell (HSC) transplantation (HSCT), we could achieve nonconditioned transplantation. Meanwhile, we found that fetal HSCs from 3 days post fertilization zebrafish embryos produce a better transplant outcome in foxn1/Casper mutants, compared with adult HSCs. In addition to HSCT, the foxn1/Casper mutant is feasible for allografts of myelodysplastic syndrome-like and muscle cells, as well as xenografts of medaka muscle cells. In summary, foxn1/Casper mutants permit the nonconditioned engraftment of multiple cell types and visualized characterization of transplanted cells in vivo.
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