4.2 Article

Validation of the G1 Molecular Marker Associated with Resistance to Orange Rust in Brazilian Sugarcane Genotypes

Journal

SUGAR TECH
Volume 23, Issue 1, Pages 108-117

Publisher

SPRINGER INDIA
DOI: 10.1007/s12355-020-00896-x

Keywords

Saccharumspp; Puccinia kuehnii; Marker-assisted selection; MAS

Categories

Funding

  1. Coordination of Superior Level Staff Improvement (CAPES/Brazil) [001]
  2. Inter-University Network for the Development of the Sugarcane Industry (RIDESA/PMGCA/UFPR)
  3. National Council for Scientific and Technological Development (CNPq/Brazil)

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The molecular marker G1 had low efficiency in predicting resistance to orange rust in Brazilian sugarcane genotypes, indicating limited effectiveness in selection for breeding programs and commercial cultivars.
Orange rust, caused by the fungusPuccinia kuehnii, is an important disease of sugarcane (Saccharumsp.). A molecular marker (G1) was developed to identify the resistance of sugarcane to orange rust. The aim of the present study was to validate the molecular marker G1 in the detection of resistance to orange rust in Brazilian sugarcane genotypes. 80 genotypes were analyzed by PCR, being classified according to the presence or absence of the marker. For field evaluation of orange rust resistance, a randomized block experiment was conducted, with the same 80 genotypes using four replications. Artificial inoculation was performed in the leaf whorl, with an evaluation made at 30 days after inoculation, using a scale from zero (without symptoms) to four (more than five pustules with sporulation), in which cultivars with an average score between zero and two were classified as resistant, and a score greater than two, susceptible. Of the 17 genotypes classified as resistant through the field test, 9 did not present the respective band. Of the 63 susceptible genotypes, 27 had the band relative to the molecular marker G1. The selection accuracy of G1 marker (the efficiency of predicting resistant phenotype) was 22.86%. The Phi correlation coefficient (phi) was calculated, through which it was observed that the variables are independent, with no statistically significant correlation between them. The results indicate that the G1 marker had a low efficiency to predict the resistance to orange rust in clones under selection in a Brazilian breeding program and Brazilian commercial cultivars of sugarcane.

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