Journal
PLOS PATHOGENS
Volume 16, Issue 10, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.ppat.1008986
Keywords
-
Categories
Funding
- National Institutes of Health [R01 AI134220]
- RNA Bioscience Initiative
- Biostatistics, Epidemiology and Research Design program of the Colorado Clinical and Translational Sciences Institute
- Deutsche Forschungsgemeinschaft [SPP 1923/1/2]
- Intramural Research Program of the National Institute of Allergy and Infectious Diseases, National Institute of Health [1ZIAAI001141]
Ask authors/readers for more resources
The Type I Interferons (IFN-Is) are innate antiviral cytokines that include 12 different IFN alpha subtypes and IFN beta that signal through the IFN-I receptor (IFNAR), inducing hundreds of IFN-stimulated genes (ISGs) that comprise the 'interferome'. Quantitative differences in IFNAR binding correlate with antiviral activity, but whether IFN-Is exhibit qualitative differences remains controversial. Moreover, the IFN-I response is protective during acute HIV-1 infection, but likely pathogenic during the chronic stages. To gain a deeper understanding of the IFN-I response, we compared the interferomes of IFN alpha subtypes dominantly-expressed in HIV-1-exposed plasmacytoid dendritic cells (1, 2, 5, 8 and 14) and IFN beta in the earliest cellular targets of HIV-1 infection. Primary gut CD4 T cells from 3 donors were treated for 18 hoursex vivowith individual IFN-Is normalized for IFNAR signaling strength. Of 1,969 IFN-regulated genes, 246 'core ISGs' were induced by all IFN-Is tested. However, many IFN-regulated genes were not shared between the IFN alpha subtypes despite similar induction of canonical antiviral ISGs such asISG15,RSAD2andMX1, formally demonstrating qualitative differences between the IFN alpha subtypes. Notably, IFN beta induced a broader interferome than the individual IFN alpha subtypes. Since IFN beta, and not IFN alpha, is upregulated during chronic HIV-1 infection in the gut, we compared core ISGs and IFN beta-specific ISGs from colon pinch biopsies of HIV-1-uninfected (n = 13) versus age- and gender-matched, antiretroviral-therapy naive persons with HIV-1 (PWH; n = 19). Core ISGs linked to inflammation, T cell activation and immune exhaustion were elevated in PWH, positively correlated with plasma lipopolysaccharide (LPS) levels and gut IFN beta levels, and negatively correlated with gut CD4 T cell frequencies. In sharp contrast, IFN beta-specific ISGs linked to protein translation and anti-inflammatory responses were significantly downregulated in PWH, negatively correlated with gut IFN beta and LPS, and positively correlated with plasma IL6 and gut CD4 T cell frequencies. Our findings reveal qualitative differences in interferome induction by diverse IFN-Is and suggest potential mechanisms for how IFN beta may drive HIV-1 pathogenesis in the gut.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available