Journal
CURRENT OPINION IN BIOTECHNOLOGY
Volume 41, Issue -, Pages 83-89Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2016.05.001
Keywords
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Funding
- European Research Council [616644]
- European Research Council (ERC) [616644] Funding Source: European Research Council (ERC)
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Stable isotope probing of RNA has enthused researchers right from its first introduction in 2002. The concept of a labelling-based detection of process-targeted microbes independent of cellular replication or growth has allowed for a much more direct handle on functionally relevant microbiota than by labelling of other biomarkers. This has led to a widespread application of the technology, and breakthroughs in our understanding of carbon flow in natural microbiomes, autotrophic and heterotrophic physiologies, microbial food webs, host-microbe interactions and environmental biotechnology. Recent studies detecting labelled mRNA demonstrate that RNA-SIP is not limited to the analysis of rRNA, but is currently developing towards an approach for accessing targeted transcriptomes. In combination with next-generation sequencing and other methodological advances, RNA-SIP will continue to deliver invaluable insights into the functioning of microbial communities.
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