Journal
CURRENT OPINION IN BIOTECHNOLOGY
Volume 41, Issue -, Pages 34-42Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2016.04.018
Keywords
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Funding
- EPSRC [EP/M002403/1]
- NERC [NE/M002934/1]
- BBSRC sLoLa [BB/M000265/1]
- NSFC [31400436]
- CAS [XDB15040100]
- ERC Advanced Grant (NITRICARE) [294343]
- Emerging Technologies Opportunity Program, Office of Science of the US Department of Energy [DE-AC02-05CH11231]
- BBSRC [BB/M000265/1] Funding Source: UKRI
- EPSRC [EP/M002403/1, EP/M02833X/1] Funding Source: UKRI
- NERC [NE/M002934/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/M002403/1, EP/M02833X/1] Funding Source: researchfish
- Natural Environment Research Council [NE/M002934/1] Funding Source: researchfish
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Microbial communities are essential for most ecosystem processes and interact in highly complex ways with virtually all eukaryotes. Thus, a detailed understanding of the function of such communities is a fundamental prerequisite for microbial ecologists, applied microbiologists and microbiome researchers. Using single cell Raman microspectroscopy, biochemical fingerprints of individual microbial cells can be obtained in an externally label-free and non-destructive manner. If combined with stable isotope probing (SIP), Raman spectroscopy can directly reveal functions of single microorganisms in their natural habitat. This review provides an update on various SIP-approaches suitable for combination with different Raman scattering techniques and illustrates how single cell Raman SIP can be directly combined with the omics-centric analysis pipelines to investigate microbial communities.
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