Journal
NUTRIENTS
Volume 12, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/nu12103157
Keywords
milk peptides; gastrointestinal digestion; Caco-2 cells; blood– brain barrier; peptide transport
Categories
Funding
- Danish Dairy Research Foundation (Aarhus N, Denmark)
- Arla Foods Ingredients Group P/S (Viby J, Denmark)
Ask authors/readers for more resources
The effect of food components on brain growth and development has attracted increasing attention. Milk has been shown to contain peptides that deliver important signals to the brains of neonates and infants. In order to reach the brain, milk peptides have to resist proteolytic degradation in the gastrointestinal tract, cross the gastrointestinal barrier and later cross the highly selective blood-brain barrier (BBB). To investigate this, we purified and characterized endogenous peptides from bovine milk and investigated their apical to basal transport by using human intestinal Caco-2 cells and primary porcine brain endothelial cell monolayer models. Among 192 characterized milk peptides, only the alpha(S1)-casein peptide (185)PIGSENSEKTTMPLW(199), and especially fragments of this peptide processed during the transport, could cross both the intestinal barrier and the BBB cell monolayer models. This peptide was also shown to resist simulated gastrointestinal digestion. This study demonstrates that a milk derived peptide can cross the major biological barriers in vitro and potentially reach the brain, where it may deliver physiological signals.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available