Journal
BIOMEDICAL OPTICS EXPRESS
Volume 11, Issue 11, Pages 6649-6658Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.404368
Keywords
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Funding
- European Research Council
- Horizon 2020 Framework Programme [678316]
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We report a cross-talk free simultaneous three-wavelength digital holographic microscopy setup for spectroscopic imaging of biological cells during flow. The feasibility of the proposed measurement technique is demonstrated on erythrocytes, due to their unique morphology and dependency of hemoglobin (Hb) molecule absorption on wavelength. From the spectroscopic quantitative phase profiles of cells acquired during flow in a microfluidic device, we decoupled the refractive index and the physical thickness. We then used our quantitative phase imaging results to dynamically calculate the mean cell volume (MCV), mean corpuscular Hb concentration (MCHC), mean corpuscular Hb content (MCH) and sphericity index. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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