Journal
BIOMEDICAL OPTICS EXPRESS
Volume 11, Issue 11, Pages 6605-6619Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.408684
Keywords
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Funding
- National Cancer Institute [P50CA097186]
- U.S. Department of Defense [W81XWH-18-10358]
- NIH [K99 CA240681, R01CA175391, R01CA244170]
- National Science Foundation [DGE1762114]
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Open-top light-sheet (OTLS) microscopes have been developed for user-friendly and versatile high-throughput 3D microscopy of thick specimens. As with all imaging modalities, spatial resolution trades off with imaging and analysis times. A hierarchical multi-scale imaging workflow would therefore be of value for many volumetric microscopy applications. We describe a compact multi-resolution OTLS microscope, enabled by a novel solid immersion meniscus lens (SIMlens), which allows users to rapidly transition between air-based objectives for low- and high-resolution 3D imaging. We demonstrate the utility of this system by showcasing an efficient 3D analysis workflow for a diagnostic pathology application. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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