Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 72, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jff.2020.104037
Keywords
Agaricus blazei; ABp-RQR; Aging; Nrf2/ARE; TLR4/NF-kappa Bp65
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Funding
- Development and Reform Commission of Jilin Province [2018C046-3]
- Science and Technology Bureau of Jilin City [201731201]
- Jilin Provincial Administration of Traditional Chinese Medicine [2019128]
- National and Local United Engineering Research and Development Center [2019128]
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Agaricus blazei is a kind of fungus plant with an edible as well as a medicinal use. Previous experiments have shown its crude peptide to possess an obvious anti-aging effect. In the present study three active components viz. ABp-RRQ, ABp-RQR and ABp-TRAR were obtained from the Agaricus blazei polypeptide. ABp-RQR was the main active component with a molecular weight of 458 Dalton, and HPLC confirmed it's a purity > 98%. When the D-galactose(D-gal)-induced NIH/3T3 cells were treated with ABp-RQR, changes were observed in the oxidative stress activities and the contents of inflammatory factors. Cell morphology and the percentage of positive cells were observed using the beta-galactosidase staining. Western blot was used to detect the expression levels of the proteins modulated in the inflammatory and antioxidant signaling pathways. Our study suggests that ABp-RQR may play an anti-aging role by alleviating the oxidative inflammatory reactions in D-gal-induced NIH/3T3 cells.
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