4.7 Article

Effects of Agaricus blazei polypeptide on cell senescence by regulation of Keap1/Nrf2/ARE and TLR4/NF-κBp65 signaling pathways and its mechanism in D-gal-induced NIH/3T3 cells

Journal

JOURNAL OF FUNCTIONAL FOODS
Volume 72, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.jff.2020.104037

Keywords

Agaricus blazei; ABp-RQR; Aging; Nrf2/ARE; TLR4/NF-kappa Bp65

Funding

  1. Development and Reform Commission of Jilin Province [2018C046-3]
  2. Science and Technology Bureau of Jilin City [201731201]
  3. Jilin Provincial Administration of Traditional Chinese Medicine [2019128]
  4. National and Local United Engineering Research and Development Center [2019128]

Ask authors/readers for more resources

Agaricus blazei is a kind of fungus plant with an edible as well as a medicinal use. Previous experiments have shown its crude peptide to possess an obvious anti-aging effect. In the present study three active components viz. ABp-RRQ, ABp-RQR and ABp-TRAR were obtained from the Agaricus blazei polypeptide. ABp-RQR was the main active component with a molecular weight of 458 Dalton, and HPLC confirmed it's a purity > 98%. When the D-galactose(D-gal)-induced NIH/3T3 cells were treated with ABp-RQR, changes were observed in the oxidative stress activities and the contents of inflammatory factors. Cell morphology and the percentage of positive cells were observed using the beta-galactosidase staining. Western blot was used to detect the expression levels of the proteins modulated in the inflammatory and antioxidant signaling pathways. Our study suggests that ABp-RQR may play an anti-aging role by alleviating the oxidative inflammatory reactions in D-gal-induced NIH/3T3 cells.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available