Journal
TRENDS IN BIOTECHNOLOGY
Volume 39, Issue 3, Pages 262-273Publisher
CELL PRESS
DOI: 10.1016/j.tibtech.2020.07.005
Keywords
-
Categories
Funding
- US Department of Energy [DE-SC0018368]
- U.S. Department of Energy (DOE) [DE-SC0018368] Funding Source: U.S. Department of Energy (DOE)
Ask authors/readers for more resources
CRISPR technology, a universal tool for genome engineering, has been revolutionizing biotechnology. The diverse Cas proteins with complex structures contain multiple evolutionarily conserved functional domains, and engineering key domains can modify the functions of CRISPR/Cas systems.
CRISPR technology is a universal tool for genome engineering that has revolutionized biotechnology. Recently identified unique CRISPR/Cas systems, as well as re-engineered Cas proteins, have rapidly expanded the functions and applications of CRISPR/Cas systems. The structures of Cas proteins are complex, containing multiple functional domains. These protein domains are evolutionarily conserved polypeptide units that generally show independent structural or functional properties. In this review, we propose using protein domains as a new way to classify protein engineering strategies for these proteins and discuss common ways to engineer key domains to modify the functions of CRISPR/Cas systems.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available