Journal
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
Volume 134, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2020.116068
Keywords
Method validation; Matrix effects; Internal standard calibration; Matrix-matched calibration; Standard addition calibration; Ionization; Signal suppression or enhancement
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The best strategy to overcome matrix effects in research is through systematic optimization by combining different approaches, while matrix effects are seldom addressed in method validation regulatory documents.
Matrix components of a sample can affect the quantitative measurement of the target analytes. This singularity is named matrix effects and should be considered for method validation procedure. Two main strategies to overcome matrix effects were suggested in the scientific literature: reducing the presence of components responsible for the effects and compensating the influence of matrix effects in the calibration methodology. It has been recommended that the best option to surpass matrix effects at research level is to establish a systematic optimization by the combination of approaches. On the other hand, from the method validation regulatory viewpoint, the information contained in the different method validation guidelines about matrix effects was uncommon. In fact, only in 54% of the evaluated regulatory documents the term matrix effects was mentioned explicitly in their contents. In addition, in the rest of the guidance documents addressing matrix effects, the subject was typically considered only superficially. (C) 2020 Elsevier B.V. All rights reserved.
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