Journal
BMC MEDICAL GENETICS
Volume 16, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s12881-015-0254-2
Keywords
Osteoarthritis; Association; Single nucleotide polymorphism; Gene expression; MCF2L; Luciferase reporter assays; Allelic expression
Categories
Funding
- Arthritis Research UK
- Medical Research Council
- Arthritis Research UK, MRC-Arthritis Research UK Centre for Integrated Research into Musculoskeletal Ageing (CIMA)
- European Union Seventh Framework Program [305815]
- NIHR Newcastle Biomedical Research Centre
- Newcastle University
- MRC [MR/K006312/1] Funding Source: UKRI
- Medical Research Council [MR/K006312/1] Funding Source: researchfish
- Versus Arthritis [20231] Funding Source: researchfish
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Background: Osteoarthritis (OA) is a painful, debilitating disease characterised by loss of articular cartilage with concurrent changes in other tissues of the synovial joint. Genetic association studies have shown that a number of common variants increase the risk of developing OA. Investigating their activity can uncover novel causal pathways and potentially highlight new treatment targets. One of the reported OA association signals is marked by the single nucleotide polymorphism (SNP) rs11842874 at chromosome 13q34. rs11842874 is positioned within a small linkage disequilibrium (LD) block within intron 4 of MCF2L, a gene encoding guanine-nucleotide exchange factor DBS. There are no non-synonymous SNPs that correlate with this association signal and we therefore set out to assess whether its effect on OA susceptibility is mediated by alteration of MCF2L expression. Methods: Nucleic acid was extracted from cartilage, synovial membrane or infrapatellar fat pad tissues from OA patients. Expression of MCF2L was measured by quantitative PCR and RNA-sequencing whilst the presence of DBS was studied using immunohistochemistry. The functional effect of SNPs within the 13q34 locus was assessed using public databases and in vitro using luciferase reporter analysis. Results: MCF2L gene and protein expression are detectable in joint tissues, with quantitative differences in the expression of the gene and in the transcript isoforms expressed between the tissues tested. There is an expression quantitative trait locus (eQTL) operating within synovial membrane tissue, with possession of the risk-conferring A allele of rs11842874 correlating with increased MCF2L expression. SNPs within the rs11842874 LD block reside within transcriptional regulatory elements and their direct analysis reveals that several show quantitative differences in regulatory activity at the allelic level. Conclusions: MCF2L is subject to a cis-acting eQTL in synovial membrane that correlates with the OA association signal. This signal contains several functional SNPs that could account for the susceptibility and which therefore merit further investigation. As far as we are aware, this is the first example of an OA susceptibility locus operating as an eQTL in synovial membrane tissue but not in cartilage.
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