4.7 Article

QTL mapping and validation of bread wheat flag leaf morphology across multiple environments in different genetic backgrounds

Journal

THEORETICAL AND APPLIED GENETICS
Volume 134, Issue 1, Pages 261-278

Publisher

SPRINGER
DOI: 10.1007/s00122-020-03695-w

Keywords

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Funding

  1. National Natural Science Foundation of China [31970243, 31971937]
  2. Key Research and Development Program of Sichuan Province [2018NZDZX0002]
  3. Applied Basic Research Programs of Science and Technology Department of Sichuan Province [2020YJ0140]
  4. Key Projects of Scientific and Technological Activities for Overseas Students of Sichuan Province

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Eight major and stably expressed QTL for flag leaf morphology were identified and validated using newly developed KASP markers in seven biparental populations with different genetic backgrounds. Two co-located intervals were identified and significant interactions for FLL- and FLW-related QTL were detected, indicating potential new loci for further study.
Key message Eight major and stably expressed QTL for flag leaf morphology across eleven environments were identified and validated using newly developed KASP markers in seven biparental populations with different genetic backgrounds. Flag leaf morphology is a determinant trait influencing plant architecture and yield potential in wheat (Triticum aestivumL.). A recombinant inbred line (RIL) population with a 55 K SNP-based constructed genetic map was used to map quantitative trait loci (QTL) for flag leaf length (FLL), width (FLW), area (FLA), angle (FLANG), opening angle (FLOA), and bend angle (FLBA) in eleven environments. Eight major QTL were detected in 11 environments with 5.73-54.38% of explained phenotypic variation. These QTL were successfully verified using the newly developed Kompetitive Allele Specific PCR (KASP) markers in six biparental populations with different genetic backgrounds. Among these 8 major QTL, two co-located intervals were identified. Significant interactions for both FLL- and FLW-related QTL were detected. Comparison analysis showed thatQFll.sau-SY-2BandQFla.sau-SY-2Bare likely new loci. Significant relationships between flag leaf- and yield-related traits were observed and discussed. Several genes associated with leaf development including the ortholog of maizeZmRAVL1, a B3-domain transcription factor involved in regulation of leaf angle, were predicted in physical intervals harboring these major QTL on reference genomes of bread wheat 'Chinese spring',T. turgidum, andAegilops tauschii. Taken together, these results broaden our understanding on genetic basis of flag leaf morphology and provide clues for fine mapping and marker-assisted breeding wheat with optimized plant architecture for promising loci.

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