Journal
STRUCTURE
Volume 28, Issue 11, Pages 1218-+Publisher
CELL PRESS
DOI: 10.1016/j.str.2020.10.001
Keywords
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Funding
- Science and Technology Innovation Committee of Shenzhen Municipality [202002073000002, JSGG 20200207155251653]
- National Institutes of Health [P50A1150481]
- UK Wellcome Trust Investigator Award [206422/Z/17/Z]
- UK Biotechnology and Biological Sciences Research Council [BB/S003339/1]
- BBSRC [BB/S003339/1] Funding Source: UKRI
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The ongoing global pandemic of coronavirus disease 2019 (COVID-19) resulted from the outbreak of SARS-CoV-2 in December 2019. Currently, multiple efforts are being made to rapidly develop vaccines and treatments to fight COVID-19. Current vaccine candidates use inactivated SARS-CoV-2 viruses; therefore, it is important to understand the architecture of inactivated SARS-CoV-2. We have genetically and structurally characterized ii-propiolactone-inactivated viruses from a propagated and purified clinical strain of SARS-CoV-2. We observed that the virus particles are roughly spherical or moderately pleiomorphic. Although a small fraction of prefusion spikes are found, most spikes appear nail shaped, thus resembling a postfusion state, where the S1 protein of the spike has disassociated from S2. Cryoelectron tomography and subtomogram averaging of these spikes yielded a density map that closely matches the overall structure of the SARS-CoV postfusion spike and its corresponding glycosylation site. Our findings have major implications for SARS-CoV-2 vaccine design, especially those using inactivated viruses.
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