Journal
SMALL
Volume 16, Issue 45, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.202002621
Keywords
antifouling properties; biosensors; electrogenerated chemiluminescence; fluorescent nanoclusters; protein hydrogels
Categories
Funding
- National Natural Science Foundation of China [21505078, 21874076]
- Natural Science Foundation of Tianjin City [18JCZDJC37800]
- Fundamental Research Funds for Central Universities (China)
- National Program for Support of Top-notch Young Professionals
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Electrochemiluminescence (ECL) showed great potential in various analytical applications, especially in the sensing of biotargets, taking advantage of its high sensitivity, selectivity, ease of spatial and temporal control, and simplified optical setup. However, during the sensing of complex biological samples, ECL sensors often suffered severe interferences from unavoidable nonspecific-binding of biomacromolecules and physical damages of ECL sensing interfaces. Herein, a hydrogel based ECL biosensing system exhibiting excellent anti-biofouling and self-healing properties is developed. A protein hydrogel composed of bovine serum albumin (BSA) directed fluorescent Au/Ag alloy nanoclusters (Au/Ag NCs) is applied in building ECL sensing systems. The hydrogel matrix facilitates the immobilization of fluorescent Au/Ag NCs as excellent ECL probes, and the porous hydrophilic structure allows the free diffusion of small molecular biotargets while rejecting macromolecular interferences. Moreover, the hydrogel exhibits excellent self-healing property, with the ECL intensity recovered rapidly in 10 min after cutting. The hydrogel ECL system is successfully applied in sensing glutathione (GSH) in serum, confirming the applicability of the hydrogel based anti-biofouling ECL sensing system in sensing complex biological samples. This research may inspire the development of novel anti-biofouling and self-healing ECL biosensors for biosensing applications.
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