Journal
SEPARATION SCIENCE AND TECHNOLOGY
Volume 56, Issue 15, Pages 2659-2666Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/01496395.2020.1835983
Keywords
B type acute lymphoblastic leukemia; immunomagnetic separation; flow cytometry; bone marrow; hematogone; microfluidic chip
Funding
- Turkiye Bilimsel ve Teknolojik Arastirma Kurumu [115E020]
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This study reported the experimental results of immunomagnetic separation efficiency of blast cells from bone marrow of pediatric leukemia patients, achieving a 94.5% capture efficiency with CD10 nano beads. The feasibility for further developments was tested by analyzing patient samples with a microfluidic chip.
In order to detect the blast cells from bone marrow of patients, one strategy is to first isolate the cells using immunomagnetic beads. The aim of this study was to report the experimental results of the immunomagnetic separation efficiency of the blast cells from bone marrow of pediatric leukemia patients. To test the efficiency of immunomagnetic separation, flow cytometry measurements at critical steps were performed. We here reported 94.5% capture efficiency for CD10 nano beads. Patients samples were also analyzed with a microfluidic chip to test the feasibility for further developments.
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