Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 328, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.128971
Keywords
Coronavirus disease 2019 (COVID-19); Post-PCR detection; 5 '-exonuclease activity; Spherical nucleic acids (SNAs); Colorimetric detection; DNA-programmable assembly
Funding
- Bu-Ali Sina University Research Council
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A colorimetric strategy based on gold nanoparticle assembly was developed for visual detection of SARS-CoV-2 RNA PCR products, simplifying the testing process and providing clear differentiation between positive and negative cases in post-PCR colorimetric test. The strategy rivals real-time PCR in sensitivity when evaluating samples from laboratory-confirmed cases of SARS-CoV-2 infection.
Continuous identification of suspected infectious cases is crucial to control the recent pandemic caused by the novel human coronavirus SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2). Real-time polymerase chain reaction (real-time PCR) technology cannot be implemented easily and in large scale in some communities due to lack of resources and infrastructures. Here, we report a simple colorimetric strategy derived from linker-based single-component assembly of gold nanoparticle-core spherical nucleic acids (AuNP-core SNAs) for visual detection of PCR products of SARS-CoV-2 ribonucleic acid (RNA) template. A palindromic linker is designed based on SARS-CoV-2 specific E gene to program the identical colloidal SNAs into large assemblies along with a distinct red-to-purple color change. The linker acts as a probe of SARS-CoV-2 RNA in conventional PCR reaction. In the presence of the correct template the palindromic linker, which is complementary to a short region within the target amplicon, is cleaved by 5'-exonuclease activity of deoxyribonucleic acid (DNA) polymerase. Cleavage of the palindromic linker during the amplification process inhibits the single-component assembly formation of SNAs. So, positive and negative viral samples produce simply red and purple colors in the post PCR colorimetric test, respectively. Evaluation of the samples obtained from cases with laboratory-confirmed SARS-CoV-2 infection revealed that our assay can rival with real-time PCR method in sensitivity.
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