Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 321, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.128502
Keywords
Electrochemical; Ratiometry; AuNPs@MoS2; Loop-mediated isothermal amplification; Ferrocene; Methylene blue
Funding
- National Natural Science Foundation of China [81873980, 31660241, 81460371]
- Science Foundation Project of Guizhou Medical and Health Commission [gzwjkj2019-1-196]
- Zunyi Science and Technology Funded Project [HZ(2019)105]
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A LAMP ratiometric electrochemical sensor (LAMP E-sensor) for the highly sensitive detection of Group B Streptococci (GBS) was developed based on recycling amplification via LAMP and a dual-signal electrochemical readout. In this LAMP E-sensor, a thiolated beta-cyclodextrin (SH-beta-CD) was immobilized onto the AuNPs@MoS2-modified electrode surface via Au-S bonding. By using a set of four specially designed primers to amplify the target gene (GBS) in less than 1 h, abundant products modified with ferrocene (Fc) were obtained. These Fcmodified products could connect beta-CD via the host-guest interaction between Fc and beta-CD. Additionally, methylene blue (MB) was embed in the duplex of the LAMP products, producing a strong electrochemical signal. Using this MB as a reporter and Fc as an internal control, the LAMP E-sensor showed a broad detection range from 1 fg mu L-1 to 100 pg mu L-1 and a lower detection limit of 0.23 fg mu L-1. Moreover, testing data from clinical samples showed a high correlation between the developed LAMP E-sensor and RT-PCR results. In conclusion, with improved sensitivity and good reproducibility, this LAMP E-sensor may be a promising alternative for GBS detection in the field of clinical pathogen-based molecular diagnostics.
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