Impact of long-term irrigation with municipal reclaimed wastewater on the uptake and degradation of organic contaminants in lettuce and leek

A two years drip irrigation of lettuce and leek crops with treated municipal wastewater without and with spiking with fourteen wastewater relevant contaminants at 10 mu g/L concentration level was conducted under greenhouse cultivation conditions to investigate their potential accumulation in soil and leaves and to assess human health related risks. Lettuce and leek crops were selected as a worse-case scenario since leafy green vegetable has a high potential for organic contaminants uptake. The results revealed limited accumulation of contaminants in soil and plant leaves, their concentration levels being in the range of 1-30 ng/g and 1-660 ng/g range in soil and leaves, respectively. This was likely related to abiotic and biotic transformation or simply binding processes in soil, which limited contaminants plant uptake. This assumption was underpinned by studies of the enantiomeric fractionation of chiral compounds (e.g. climbazole and metoprolol) in soil as pieces of evidence of biodegradation and by the identification of transformation products or metabolites in leaves by means of liquid chromatography - high resolution - mass spectrometry using a suspect screening workflow. The high bioconcentration factors were not limited to compounds with intermediate D-ow (100 to 1000) such as carbamazepine but also observed for hydrophilic compounds such as clarithromycin, hydrochlorothiazide and the food additives acesulfame and sucralose. This result assumed that accumulation was not only driven by passive processes (e.g. lipoidal diffusion through lipid bilayer cell membranes or Casparian strip) but might be supported by carrier-mediated transporters. As a whole, this study confirmed earlier reports on the a de minimis human health risk related to the consumption of raw leafy green vegetable irrigated with domestic TWW containing organic contaminants residues. (C) 2020 Elsevier B.V. All rights reserved.

Automated summary

The study found limited accumulation of contaminants in soil and plant leaves, likely due to transformation or binding processes in soil limiting contaminant uptake by plants. This suggests a minimal human health risk from consuming raw leafy green vegetables irrigated with treated municipal wastewater containing organic contaminants residues.