Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 735, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.scitotenv.2020.139496
Keywords
Endocrine disruption; Testosterone; Estradiol; Vitellogenin; Testes; Ovary
Categories
Funding
- Council of Scientific and Industrial Research -Human Resource Development Group (CSIR -HRDG), Government of India
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Pyriproxyfen (PPF), a broad-spectrum insecticide known to cause reproductive and endocrine disruption in invertebrates, while the data is scarce in aquatic vertebrates. The goal of this study is to investigate the impact of PPF on reproductive endocrine system of male and female zebrafish along hypothalamus-pituitary-gonadal (HPG) axis. In brain, PPF caused significant alteration in the transcripts of era, lh beta, and cyp19b genes in male and fsh beta, lh beta, and cyp19b genes in female zebrafish. The downstream genes of steroidogenic pathway like, star, 3 beta hsd, 17 beta hsd, and cyp19a expression were significantly altered in gonad of both sexes. Subsequent changes in circulatory steroid hormone levels lead to imbalance in hormone homeostasis as revealed from estradiol/testosterone (E-2/T) ratio. Further, the vitellogenin transcript level was enhanced in hepatic tissues and their blood plasma content was increased in male (16.21%) and declined in female (21.69%). PPF also induced histopathological changes in gonads such as, reduction of mature spermatocytes in male and vitellogenic oocytes in female zebrafish. The altered E-2/T ratio and gonadal histopathology were supported by the altered transcript levels of HPG axis genes. Overall, these findings provide new insights of PPF in zebrafish reproductive system and highlights for further investigations on its potential risks in aquatic environment.
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