Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 117, Issue 44, Pages 27148-27153Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2010136117
Keywords
microbial adhesion; plasmonic imaging; interfacial force; biofilm formation
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Funding
- National Natural Science Foundation of China [NSFC 21676260, 51722810, 51821006]
- National Key Research and Development Program of China [2019YFC1905002]
- Fundamental Research Funds for the Central Universities
- Shandong Key Laboratory of Water Pollution Control and Resource Reuse [2019KF02]
- Key Research and Development Program of Anhui Province [1804a0802191]
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Probing the binding between a microbe and surface is critical for understanding biofilm formation processes, developing biosensors, and designing biomaterials, but it remains a challenge. Here, we demonstrate a method to measure the interfacial forces of bacteria attached to the surface. We tracked the intrinsic fluctuations of individual bacterial cells using an interferometric plasmonic imaging technique. Unlike the existing methods, this approach determined the potential energy profile and quantified the adhesion strength of single cells by analyzing the fluctuations. This method provides insights into biofilm formation and can also serve as a promising platform for investigating biological entity/surface interactions, such as pathogenicity, microbial cell capture and detection, and antimicrobial interface screening.
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