Journal
PLANT SCIENCE
Volume 299, Issue -, Pages -Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2020.110611
Keywords
Apple; WRKY transcription factor; Salt stress; Osmotic stress; Functional analysi
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Funding
- National Key Research and Development Program of China [2018YFD1000303]
- earmarked fund for the China Agriculture Research System [CARS-27]
- National Natural Science Foundation of China [31401852, 31701894]
- Natural Science Basic Research Plan in Shaanxi Province of China [2018JQ3010]
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Abiotic stresses threaten the productivity and quality of economically important perennial fruit crops such as apple (Malus x domestica Borkh.). WRKY transcription factors play various roles in plant responses to abiotic stress, but little is known regarding WRKY genes in apple. Here, we carried out functional characterization of an apple Group IIa WRKY gene (MdWRKY30). qRT-PCR analysis found that MdWRKY30 expression was induced by salt and drought stress. A subcellular localization assay showed that MdWRKY30 is localized to the nucleus. A transactivation assay found that MdWRKY30 has no transcriptional activation activity. A Y2H assay indicated that MdWRKY26, MdWRKY28, and MdWRKY30 interact with each other to form heterodimers and homodimers. Transgenic analysis revealed that the overexpression of MdWRKY30 in Arabidopsis enhanced salt and osmotic tolerance in the seedling stage, as well as during the seed germination and greening cotyledon stages. MdWRKY30 overexpression enhanced tolerance to salt and osmotic stresses in transgenic apple callus through transcriptional regulation of stress-related genes. Together, our results demonstrate that MdWRKY30 is an important regulator of salinity and osmotic stress tolerance in apple.
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