Comparative proteomics reveals new insights into the endosperm responses to drought, salinity and submergence in germinating wheat seeds

Key Message Beyond the role of a nutrient reservoir during germination, the endosperm of wheat seeds also responds to different abiotic stresses via modification of the protein profiles. The endosperm is the main component of wheat seeds. During seed germination, it provides nutrients to support the embryo development, and its constituents vary under environmental stresses such as drought, salinity and submergence that are associated with disordered water supply. However, the molecular mechanism of these stress responses remains unclear. In this study, a comparative label-free proteomic analysis was performed on endosperm from the germinating wheat seeds subjected to PEG, NaCl and submergence treatments. In total, 2273 high confidence proteins were detected, and 234, 207 and 209 of them were identified as differentially expressed proteins (DEPs) under the three stresses, respectively. Functional classification revealed that the DEPs were mainly involved in protein, amino acid and organic acid metabolic process in all stress treatments. While some other metabolic processes were highlighted in one or two of the stresses specifically, such as oxidative phosphorylation in PEG and submergence, and beta-alanine metabolism in PEG and NaCl treatments. The identification of a series of stress-related proteins and their biased expression in different stresses indicates the active stress-responding role of endosperm beyond a simple nutrient reservoir during germination, while the overall stress responses of the endosperm were found to be moderate and lag behind the embryo. Besides, some fundamental processes and DEPs shared by the three stresses could be selected priorly for future molecular breeding researches. Our results provide new insights into the mechanism of endosperm responses to abiotic stresses during seed germination.

Automated summary

The endosperm of wheat seeds plays a role as a nutrient reservoir during germination and responds to different abiotic stresses through modification of protein profiles. Functional classification of differentially expressed proteins (DEPs) under PEG, NaCl and submergence treatments revealed their involvement in protein, amino acid and organic acid metabolic processes, with specific metabolic pathways highlighted in each stress condition. The identification of stress-related proteins and their biased expression in different stresses suggests an active stress-responding role of endosperm beyond nutrient storage during germination.