Journal
PLANT MOLECULAR BIOLOGY
Volume 104, Issue 4-5, Pages 397-410Publisher
SPRINGER
DOI: 10.1007/s11103-020-01047-2
Keywords
Abiotic stress; Potassium deficiency; JAZ repressor; Root system architecture; Lateral roots
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Funding
- UGC
- CSIR
- DBT
- INSA-young scientist project
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Key message Enhanced bioactive JA (JA-Ile) accumulation in OsJAZ9 overexpressing rice helps plants tolerate K deficiency. Potassium (K) represents up to 10% of the plant's total dry biomass, and its deficiency makes plants highly susceptible to both abiotic and biotic stresses. K shortage results in the inhibition of root and shoots growth, but the underlying mechanism of this response is unclear. Our RNA-Seq and qPCR analysis suggested leading roles for JA pathway genes under K deficiency in rice. Notably, K deficiency and JA application produced similar phenotypic and transcriptional responses. Here, we integrated molecular, physiological and morphological studies to analyze the role ofOsJAZ9in JA homeostasis and K deficiency responses. We raisedOsJAZ9over-expression, knockdown, transcriptional reporter, translational reporter and C-terminal deleted translational reporter lines in rice to establish the role of JA signaling in K ion homeostasis. JA profiling revealed significantly increased JA-Ile levels inOsJAZ9OE lines under K deficiency. Furthermore, we established thatOsJAZ9overexpression and knockdown result in K deficiency tolerance and sensitivity, respectively, by modulating various K transporters and root system architecture. Our data provide evidence on the crucial roles ofOsJAZ9for improving K deficiency tolerance in rice by altering JA levels and JA responses.
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