Abscisic acid in preservation ofTaraxacum pieninicumin the form of synthetic seeds in slow growth conditions

Taraxacum pieninicumPawl. is listed as critically endangered species, for which currently applied protection methods are insufficient. The aim of this study was to investigate the possibility ofT. pieninicumstorage in the form of synthetic seeds under slow-growth conditions in combination with ABA treatment, as one of the ex situ protection methods of this species. The obtained results indicated that darkness was much more favorable condition for synseed storage and did not generate additional stress during cold exposure in contrast to the light conditions. The preculture of shoot tips on the medium supplemented with ABA led to a decrease in the shoots proliferation rate and inhibition of their growth. ABA clearly inhibited growth of the encapsulated shoot tips also during cold storage. Biochemical parameters showed that ABA effectively reduced the negative effect of the cold stress, what was found on the basis of analyzes of H(2)O(2)and TBARS levels in the stored material. Moreover, synseeds stored under light conditions and treated with ABA exhibited decreased level of endogenous jasmonic acid what indicated interaction between those two phytohormones at a low temperature. The study also demonstrated that in vitro culture, cold storage and ABA treatment had no effect on the flowering process of this species after acclimatization to ex vitro conditions. Key message The aim of this research was to assess ABA effect on post-storage regrowth and the stress factors level in plants obtained after long-term storage ofTaraxacum pieninicumsynthetic seeds.

Automated summary

This study demonstrates the potential of storing Taraxacum pieninicum as synthetic seeds under slow-growth conditions with ABA treatment. Results show that darkness is more favorable for synseed storage and ABA treatment can effectively reduce the negative effects of cold stress on the plant.