Journal
PLANT BIOLOGY
Volume 23, Issue 2, Pages 351-362Publisher
WILEY
DOI: 10.1111/plb.13175
Keywords
Phytoremediation; proteomics; photosynthesis; ascorbic acid; proline; Phragmites australis
Categories
Funding
- National Natural Science Foundation of China [51608246]
- Open Project of State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology [QA201715]
Ask authors/readers for more resources
The study found that under copper stress, Phragmites australis showed decreased chlorophyll content, inhibited expression of important photosynthesis proteins, and reduced photosynthesis. However, the levels of ascorbic acid and proline increased, limiting copper migration within the plant. These findings provide insight into the tolerance and repair mechanisms of plants in response to heavy metal pollution.
Few relevant research attempts have been made to determine heavy metal resistance mechanisms of rhizomatous perennial plants. Thus, it is pertinent to investigate the physiological and biochemical changes in Phragmites australis under metal-stressed conditions to facilitate the development of strategies to enhance copper (Cu) tolerance. We measured parameters related to plant growth and development, metal translocation and physiological responses of P. australis subjected to Cu stress. In addition, the differentially expressed proteins (DEP) were evaluated using the isobaric tag for relative and absolute quantification (iTRAQ) system. A large amount of copper accumulates in the roots of P.australis, but the growth parameters were not sensitive to Cu. However, the high concentration of Cu reduced the content of chlorophyll a and chlorophyll b, and the expression of important photosynthesis proteins PsbD, PsbO and PsaA were all down-regulated, so photosynthesis was inhibited. In contrast, the content of ascorbic acid and proline both increased with the increase of copper stress. P.australis fixed a large amount of Cu in its roots, limiting the migration of Cu to other parts of the plant. Moreover, Cu stress can affect photosynthesis by inhibiting the activity of PSI, PSII and LHCII. In addition, P.australis synthesizes ascorbic acid through the D-mannose/L-galactose pathway, and synthesizes proline through the ornithine pathway. Ascorbic acid and proline can increase Cu tolerance and protect photosynthesis. These results provide a theoretical basis for understanding the tolerance and repair mechanisms of plants in response to heavy metal pollution.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available