Journal
NEW PHYTOLOGIST
Volume 229, Issue 2, Pages 820-830Publisher
WILEY
DOI: 10.1111/nph.16912
Keywords
apoplastic pH; embolism; Populus; recovery; sugars; vanadate; X-ray microcomputed tomography (micro-CT); xylem
Categories
Funding
- Ateneo CSP/2016 Project (University of Turin)
Ask authors/readers for more resources
Research shows that in drought-stressed plants, a coordinated cascade of chemical and transcriptional adjustments occurs alongside embolism formation, affecting the hydraulic recovery process. These adjustments, including pH modification and sugar concentration increase, prime the stems for rehydration.
In drought-stressed plants a coordinated cascade of chemical and transcriptional adjustments occurs at the same time as embolism formation. While these processes do not affect embolism formation during stress, they may prime stems for recovery during rehydration by modifying apoplast pH and increasing sugar concentration in the xylem sap. Here we show thatin vivotreatments modifying apoplastic pH (stem infiltration with a pH buffer) or reducing stem metabolic activity (infiltration with sodium vanadate and sodium cyanide; plant exposure to carbon monoxide) can reduce sugar accumulation, thus disrupting or delaying the recovery process. Application of the vanadate treatment (NaVO(3,)an inhibitor of many ATPases) completely halted recovery from drought-induced embolism for up to 24 h after re-irrigation, while partial recovery was observedin vivoin control plants using X-ray microcomputed tomography. Our results suggest that stem hydraulic recovery in poplar is a biological, energy-dependent process that coincides with accumulation of sugars in the apoplast during stress. Recovery and damage are spatially coordinated, with embolism formation occurring from the inside out and refilling from the outside in. The outside-in pattern highlights the importance of xylem proximity to the sugars within the phloem to the embolism recovery process.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available