4.5 Article

The condensin holocomplex cycles dynamically between open and collapsed states

Journal

NATURE STRUCTURAL & MOLECULAR BIOLOGY
Volume 27, Issue 12, Pages 1134-U112

Publisher

NATURE PORTFOLIO
DOI: 10.1038/s41594-020-0508-3

Keywords

-

Funding

  1. ERC [669598, 753002, 681365]
  2. Netherlands Organization for Scientific Research (NWO/OCW) as part of the Frontiers of Nanoscience and Basyc programs
  3. European Molecular Biology Laboratory
  4. Marie Curie Actions (MSCA) [753002] Funding Source: Marie Curie Actions (MSCA)
  5. European Research Council (ERC) [681365] Funding Source: European Research Council (ERC)

Ask authors/readers for more resources

Atomic force microscopy imaging of yeast condensin indicates that condensin may extrude DNA by switching conformation between open O and collapsed B shapes, indicative of a type of scrunching model. Structural maintenance of chromosome (SMC) protein complexes are the key organizers of the spatiotemporal structure of chromosomes. The condensin SMC complex has recently been shown to be a molecular motor that extrudes large loops of DNA, but the mechanism of this unique motor remains elusive. Using atomic force microscopy, we show that budding yeast condensin exhibits mainly open 'O' shapes and collapsed 'B' shapes, and it cycles dynamically between these two states over time, with ATP binding inducing the O to B transition. Condensin binds DNA via its globular domain and also via the hinge domain. We observe a single condensin complex at the stem of extruded DNA loops, where the neck size of the DNA loop correlates with the width of the condensin complex. The results are indicative of a type of scrunching model in which condensin extrudes DNA by a cyclic switching of its conformation between O and B shapes.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available