Journal
NATURE METHODS
Volume 17, Issue 9, Pages 909-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-020-0918-5
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Funding
- European Research Council [ERC CoG-724489]
- National Institutes of Health Common Fund 4D Nucleome Program [U01 EB021223]
- Human Frontier Science Program [RGY0065/2017]
- Engelhorn Foundation (Postdoctoral Fellowship)
- European Molecular Biology Laboratory
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High laser powers are common practice in single-molecule localization microscopy to speed up data acquisition. Here we systematically quantified how excitation intensity influences localization precision and labeling density, the two main factors determining data quality. We found a strong trade-off between imaging speed and quality and present optimized imaging protocols for high-throughput, multicolor and three-dimensional single-molecule localization microscopy with greatly improved resolution and effective labeling efficiency.
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