Journal
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Volume 29, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.nano.2020.102260
Keywords
DNA nanotechnology; Drug delivery; Ophthalmology; Nucleic acids; Nanoparticles; Glaucoma; Aptamers
Funding
- EXIST research transfer program of the Federal Ministry for Economic Affairs and Energy Germany [03EFDBW075]
- Zernike Institute for Advanced Materials
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Lipid DNA nanoparticles (NPs) exhibit an intrinsic affinity to the ocular surface and can be loaded by hybridization with fluorophore-DNA conjugates or with the anti-glaucoma drug travoprost by hybridizing an aptamer that binds the medication. In the travoprost-loaded NPs (Trav-NPs), the drug is bound by specific, non-covalent interactions, not requiring any chemical modification of the active pharmaceutical ingredient. Fluorescently labeled Trav-NPs show a long-lasting adherence to the eye, up to sixty minutes after eye drop instillation. Biosafety of the Trav-NPs was proved and in vivo. Ex vivo and in vivo quantification of travoprost via LC-MS revealed that Trav-NPs deliver at least twice the amount of the drug at every time-point investigated compared to the pristine drug. The data successfully show the applicability of a DNA-based drug delivery system in the field of ophthalmology for the treatment of a major retinal eye disease, i.e. glaucoma. (C) 2020 Elsevier Inc. All rights reserved.
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