Functionalized graphene oxide nanosheets with unique three-in-one properties for efficient and tunable antibacterial applications

Developing antibiotics-independent antibacterial agents is of great importance since antibiotic therapy faces great challenges from drug resistance. Graphene oxide (GO) is a promising agent due to its natural antibacterial mechanisms, such as sharp edge-mediated cutting effect. However, the antibacterial activity of GO is limited by its negative charge and low photothermal effect. Herein, the amino-functionalized GO nanosheets (AGO) with unique three-in-one properties were synthesized. Three essential properties (positive charge, strong photothermal effect, and natural cutting effect) were integrated into AGO. The positive charge (30 mV) rendered AGO a strong interaction force with model pathogenStreptococcus mutans(330 nN). The natural cutting effect of 100 mu g.mL(-1)AGO caused 27% loss of bacterial viability after incubation for 30 min. Most importantly, upon the near-infrared irradiation for just 5 min, the three-in-one properties of AGO caused 98% viability loss. In conclusion, the short irradiation period and the tunable antibacterial activity confer the three-in-one AGO a great potential for clinical use.

Automated summary

Amino-functionalized graphene oxide nanosheets (AGO) with three-in-one properties, including positive charge, strong photothermal effect, and natural cutting effect, show strong interaction with model pathogen Streptococcus mutans and cause significant bacterial cell death under near-infrared irradiation.