Journal
NANO LETTERS
Volume 20, Issue 10, Pages 6957-6965Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.0c01297
Keywords
Molecular assembly; supramolecular polymers; protein purification; monoclonal antibody; peptides
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Funding
- Bristol-Myers Squibb
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The separation and purification of therapeutic proteins from their biological resources pose a great limitation for industrial manufacturing of biologics in an efficient and cost-effective manner. We report here a supramolecular polymeric system that can undergo multiple reversible processes for efficient capture, precipitation, and recovery of monoclonal antibodies (mAbs). These supramolecular polymers, namely immunofibers (IFs), are formed by coassembly of a mAb-binding peptide amphiphile with a rationally designed filler molecule of varying stoichiometric ratios. Under the optimized conditions, IFs can specifically capture mAbs with a precipitation yield greater than 99%, leading to an overall mAb recovery yield of 94%. We also demonstrated the feasibility of capturing and recovering two mAbs from clarified cell culture harvest. These results showcase the promising potential of peptide-based supramolecular polymers as reversible affinity precipitants for mAb purification.
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