4.6 Article

A Nuclear Magnetic Resonance (NMR) Platform for Real-Time Metabolic Monitoring of Bioprocesses

Journal

MOLECULES
Volume 25, Issue 20, Pages -

Publisher

MDPI
DOI: 10.3390/molecules25204675

Keywords

NMR-compatible bioreactor; automated NMR; bioprocess monitoring; microbial bioprocess; waste degradation

Funding

  1. project BioPEC - Federal Ministry of Education and Research (BMBF), Germany [01DQ17014]
  2. project BioPEC - Department of Biotechnology (DBT), India [BT/IN/BMBF-Germany/29/SKM/2016-17]
  3. KIT-Publication Fund of the Karlsruhe Institute of Technology

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We present a Nuclear Magnetic Resonance (NMR) compatible platform for the automated real-time monitoring of biochemical reactions using a flow shuttling configuration. This platform requires a working sample volume of similar to 11 mL and it can circulate samples with a flow rate of 28 mL/min, which makes it suitable to be used for real-time monitoring of biochemical reactions. Another advantage of the proposed low-cost platform is the high spectral resolution. As a proof of concept, we acquire 1H NMR spectra of waste orange peel, bioprocessed using Trichoderma reesei fungus, and demonstrate the real-time measurement capability of the platform. The measurement is performed over more than 60 h, with a spectrum acquired every 7 min, such that over 510 data points are collected without user intervention. The designed system offers high resolution, automation, low user intervention, and, therefore, time-efficient measurement per sample.

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