Journal
MOLECULES
Volume 25, Issue 19, Pages -Publisher
MDPI
DOI: 10.3390/molecules25194507
Keywords
Ceratophyllum demersum L; response surface methodology; α -glucosidase inhibitors; phytochemical profile
Funding
- National Key R&D Program of China [2018YFD0901101]
- Open Project Program of State Key Laboratory of Food Science and Technology, Nanchang University [SKLF-KF-201804]
- National Natural Science Foundation of China [31860475]
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Ceratophyllum demersum L. (CDL) is a traditional Chinese herb to treat many diseases, but research on its anti-diabetic activity is not available. In this research, the alpha-glucosidase inhibitory ability and phytochemical constituents of CDL extract were firstly studied. Optimal ultrasound-assisted extraction conditions for alpha-glucosidase inhibitors (AGIs) were optimized by single factor experiment and response surface methodology (RSM), which was confirmed as 70% methanol, liquid-to-solid ratio of 43 (mL/g), extraction time of 54 min, ultrasonic power of 350 W, and extraction temperature of 40 degrees C. The lowest IC50 value for alpha-glucosidase inhibition was 0.15 mg dried material/mL (mg DM/mL), which was much lower than that of acarbose (IC50 value of 0.64 mg DM/mL). In total, 80 compounds including 8 organic acids, 11 phenolic acids, 25 flavonoids, 21 fatty acids, and 15 others were identified or tentatively identified from CDL extract by HPLC-QTOF-MS/MS analysis. The results suggested that CDL could be a potential source of alpha-glucosidase inhibitors. It can also provide useful phytochemical information for research into other bioactivities.
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