Journal
MOLECULES
Volume 25, Issue 18, Pages -Publisher
MDPI
DOI: 10.3390/molecules25184332
Keywords
biosynthesis; silver nanoparticles; MCF-7; MCF-7; TAMR-1; MCF-10A; cytotoxicity; apoptosis
Funding
- Fundamental Research Grant Scheme by Ministry of Higher Education of Malaysia [203.CIPPT.6711601]
- Universiti Sains Malaysia [304/CIPPT/6313297]
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A bottom-up approach for synthesizing silver nanoparticles (AgNPs-GA) phytomediated byGarcinia atroviridisleaf extract is described. Under optimized conditions, the AgNPs-GA were synthesized at a concentration of 0.1 M silver salt and 10% (w/v) leaf extract, 1:4 mixing ratio of reactants, pH 3, temperature 32 degrees C and 72 h reaction time. The AgNPs-GA were characterized by various analytical techniques and their size was determined to be 5-30 nm. FTIR spectroscopy indicates the role of phenolic functional groups in the reduction of silver ions into AgNPs-GA and in supporting their subsequent stability. The UV-Visible spectrum showed an absorption peak at 450 nm which reflects the surface plasmon resonance (SPR) of AgNPs-GA and further supports the stability of these biosynthesized nanoparticles. SEM, TEM and XRD diffractogram analyses indicate that AgNPs-GA were spherical and face-centered-cubic in shape. This study also describes the efficacy of biosynthesized AgNPs-GA as anti-proliferative agent against human breast cancer cell lines, MCF-7 and MCF-7/TAMR-1. Our findings indicate that AgNPs-GA possess significant anti-proliferative effects against both the MCF-7 and MCF-7/TAMR-1 cell lines, with inhibitory concentration at 50% (IC(50)values) of 2.0 and 34.0 mu g/mL, respectively, after 72 h of treatment. An induction of apoptosis was evidenced by flow cytometry using Annexin V-FITC and propidium iodide staining. Therefore, AgNPs-GA exhibited its anti-proliferative activity via apoptosis on MCF-7 and MCF-7/TAMR-1 breast cancer cells in vitro. Taken together, the leaf extract fromGarcinia atroviridiswas found to be highly capable of producing AgNPs-GA with favourable physicochemical and biological properties.
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