Journal
MOLECULAR PLANT PATHOLOGY
Volume 21, Issue 12, Pages 1620-1633Publisher
WILEY
DOI: 10.1111/mpp.13002
Keywords
Arabidopsis thaliana; Botrytis cinerea; cell wall integrity; pectin methylesterases; plant immunity
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Funding
- Agence Nationale de la Recherche
- Regione Lazio [ABASA CUPB81G18000770002]
- Sapienza Universita di Roma [RM11816432F244FD, RM11916B7A142CF1, RP1181642E99296A]
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Pectin is synthesized in a highly methylesterified form in the Golgi cisternae and partially de-methylesterified in muro by pectin methylesterases (PMEs).Arabidopsis thalianaproduces a local and strong induction of PME activity during the infection of the necrotrophic fungusBotrytis cinerea. AtPME17 is a putativeA. thalianaPME highly induced in response toB. cinerea. Here, a fine tuning of AtPME17 expression by different defence hormones was identified. Our genetic evidence demonstrates that AtPME17 strongly contributes to the pathogen-induced PME activity and resistance againstB. cinereaby triggering jasmonic acid-ethylene-dependentPDF1.2expression. AtPME17 belongs to group 2 isoforms of PMEs characterized by a PME domain preceded by an N-terminal PRO region. However, the biochemical evidence for AtPME17 as a functional PME is still lacking and the role played by its PRO region is not known. Using thePichia pastorisexpression system, we demonstrate that AtPME17 is a functional PME with activity favoured by an increase in pH. AtPME17 performs a blockwise pattern of pectin de-methylesterification that favours the formation of egg-box structures between homogalacturonans. Recombinant AtPME17 expression inEscherichia colireveals that the PRO region acts as an intramolecular inhibitor of AtPME17 activity.
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