Journal
MOLECULAR CARCINOGENESIS
Volume 59, Issue 10, Pages 1199-1208Publisher
WILEY
DOI: 10.1002/mc.23249
Keywords
breast cancer; lncRNA CERS6-AS1; miR-125a-5p
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Funding
- National Natural Science Foundation of China [81802651, 81671586]
- Key Research Foundation of Wannan Medical College [WK2019Z7]
- Foundation of Peak Training Program for Scientific Research of Yijishan Hospital, Wannan Medical College [GF2019G11]
- Foundation for the Cultivation of Outstanding and Top Talents in Colleges and Universities of Anhui Province [gxgnfx2019010]
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Long noncoding RNAs (lncRNAs) can act as oncogene and tumor suppressor genes in many types of cancers including breast cancer (BC). Our previous study has indicated microRNA (miR)-125a-5p was downregulated and function as a tumor suppressor in BC. However, its upstream regulation mechanism is still unclear. In this study, we used bioinformatics algorithms, RNA pulldown assay, and dual-luciferase reports assay to predict and confirm lncRNA CERS6-AS1 interacted with miR-125a-5p. Then we found CERS6-AS1 was upregulated in BC tissues. Experimental results of tumor growth in nude mice show that CERS6-AS1 promotes tumor growth. Furthermore, CERS6-AS1 regulated BC susceptibility gene 1-associated protein 1 (BAP1) expression via sponging miR-125a-5p via Western blot analysis and quantitative polymerase chain reaction arrays. Finally, we showed that miR-125a-5p had opposing effects to those of CERS6-AS1 on BC cells, demonstrating that CERS6-AS1 may promote cell proliferation and inhibit cell apoptosis via sponging miR-125a-5p. Our results indicated CERS6-AS1 promote BC cell proliferation and inhibit cell apoptosis via sponging miR-125a-5p to upregulate BAP1 expression.
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