Journal
MOLECULAR AND CELLULAR PROBES
Volume 54, Issue -, Pages -Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2020.101669
Keywords
Recombinase polymerase amplification; Feline coronavirus; Detection
Categories
Funding
- Yibin University, China [2019QD09, 2019QD10]
- Science and Technology Planning Project of Guangdong Province [2018B030317001]
- Province prevention and control novel coronavirus infection of Guangdong Province, Guangzhou, China [2020A111128021]
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Feline coronavirus (FCoV) is classified into two pathotypes: the avirulent feline enteric coronavirus (FECV), and the virulent feline infectious peritonitis virus (FIPV). Rapid pathogen detection, which is efficient and convenient, is the best approach for early confirmatory diagnosis. In this study, we first developed and evaluated a rapid recombinase polymerase amplification (RPA) detection method for FCoV that can detect FCoV within 15 min at 39 degrees C. The detection limit of that assay was 233 copies/mu L DNA molecules per reaction. The specificity was high: it did not cross-react with canine distemper virus (CDV), canine coronavirus (CCoV), canine adenovirus (CAV), feline calicivirus (FCV), feline herpesvirus (FHV), or feline parvovirus (FPV). This assay was evaluated using 42 clinical samples (30 diarrhea samples and 12 ascites samples). The coincidence rate between FCoV-RPA and RT-qPCR for detection in clinical samples was 95.2%. In summary, FCoV-RPA analysis provides an efficient, rapid, and sensitive detection method for FCoV.
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