Journal
MOLECULAR & CELLULAR PROTEOMICS
Volume 19, Issue 12, Pages -Publisher
ELSEVIER
DOI: 10.1074/mcp.RA120.002251
Keywords
proteomics; spermatozoa; domestic cat; epididymis; extracellular vesicles; biofluids; mass spectrometry; molecular biology; protein identification; secretome; domestic cat; epididymis; extracellular vesicles; proteomics; spermatozoa
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Funding
- Smithsonian's Scholarly Studies Program
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Extracellular vesicles (EVs) secreted by the epididymal epithelium transfer to spermatozoa key proteins that are essential in promoting motility and subsequent fertilization success. Using the domestic cat model, the objectives were to (1) characterize and compare protein content of EVs between segments of the epididymis, and (2) compare EV protein compositions between normo- and teratospermic individuals (producing >60% of abnormal spermatozoa). Epididymal EVs from adult cats were isolated and assessed via liquid chromatography tandem MS. Both male types shared 3008 proteins in total, with 98 and 20 EV proteins unique to normospermic and teratospermic males, respectively. Expression levels of several proteins changed between epididymal segments in both male types. Several proteins in both groups were related to sperm motility (e.g. hexokinase 1, adenylate kinase isoenzyme) and zona pellucida or oolemma binding (e.g. disintegrin and metalloproteinase domain proteins, zona binding proteins 1 and 2). Interestingly, seven cauda-derived EV proteins trended downward in teratospermic compared with normospermic males, which may relate to poor sperm quality. Collective results revealed, for the first time, EV proteins related to sequential sperm maturation with differences observed between normospermic and teratospermic individuals. Extracellular vesicles (EVs) isolated from epididymal segments of both normospermic and teratospermic domestic cats were analyzed via mass spectrometry. Both male types shared 3008 proteins, with 98 and 20 EV proteins unique to normospermic and teratospermic males, respectively. Expression levels of several proteins changed between epididymal segments in both male types, with seven cauda-derived proteins trending downward in teratospermic compared to normospermic males. Collectively, these differences may relate to the poor sperm quality observed in teratospermic individuals.
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