Journal
MICROVASCULAR RESEARCH
Volume 131, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.mvr.2020.104031
Keywords
Single-cell; Choroid; Choriocapillaris; Pericytes; Infant; Age-related macular degeneration
Categories
Funding
- NIH [T32 GM007337, EY024605, P30 EY025580]
- Research to Prevent Blindness
- Elmer and Sylvia Sramek Charitable Trust
- Martin Carver Chair in Ocular Cell Biology
- NATIONAL EYE INSTITUTE [F30EY031923] Funding Source: NIH RePORTER
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The human choroidal vasculature is subject to age-related structural and gene expression changes implicated in age-related macular degeneration (AMD). In this study, we performed both bulk and single-cell RNA sequencing on infant (n = 4 for bulk experiments, n = 2 for single-cell experiments) and adult (n = 13 for bulk experiments, n = 6 for single-cell experiments) human donors to characterize how choroidal gene expression changes with age. Differential expression analysis revealed that aged choroidal samples were enriched in genes encoding proinflammatory transcription factors and leukocyte transendothelial cell migration adhesion proteins. Such genes were observed to be differentially expressed specifically within choroidal endothelial cells at the single-cell level. Immunohistochemistry experiments support transcriptional findings that CD34 is elevated in infant choriocapillaris endothelial cells while ICAM-1 is enriched in adults. These results suggest several potential drivers of the pro-inflammatory vascular phenotype observed with advancing age.
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