Journal
MICROCHIMICA ACTA
Volume 187, Issue 10, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-020-04546-7
Keywords
Distance-dependent visual immunoassay; Silver ion-exchange reaction; Microfluidic paper-based analytical device; CdTe quantum dots; Silver nanolabels; Matrix metalloproteinase-7
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Funding
- National Natural Science Foundation of China [21675029, 201874022]
- Health-Education Joint Research Project of Fujian Province [WKJ2016-2-15]
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A paper-based visual fluorescence immunoassay is presented for the detection of matrix metalloproteinase-7 (MMP7) that is related to renal cancer. The method is based on the distance-dependent fluorescence quenching of CdTe quantum dots (QDs) on a nitrocellulose membrane by Ag+ following a sandwich-type immunoreaction on microtiter wells using silver nanoparticle (AgNP)-labeled secondary antibody- and primary antibody-coated microtiter wells. The silver nanoparticles captured in the well are dissolved with HNO3, while the quenching effect of QDs is based on silver ion-exchange reaction under 365-nm excitation light irradiation. Increasing concentration of released Ag+, thus higher concentration of the protein, leads to an increased distance of quenching on the nitrocellulose membrane. The paper-based immunoassay by combination of AgNP-assisted ion-exchange reaction with QD gives good distance-dependent responses and allows the detection of MMP7 at a concentration as low as 7.3 pg mL(-1). The coefficients of variation are less than 6.9% and 12.4% for intra-assay and inter-assay, respectively. High specificity and long-term stability are achieved during the assay. Importantly, the testing of human serum samples using our strategy shows well-matched results with commercial human MMP7 ELISA
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