Journal
MICROBIOLOGICAL RESEARCH
Volume 239, Issue -, Pages -Publisher
ELSEVIER GMBH
DOI: 10.1016/j.micres.2020.126539
Keywords
Toxin-antitoxin systems; Clostridioides difficile; Heterologous expression
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Funding
- Agencia Nacional de Investigacion y Desarrollo (FONDECYT Grant) [1171397]
- Millennium Science Initiative of the Ministry of Economy, Development, and Tourism, grant Nucleus in the Biology of Intestinal Microbiota
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Clostridioides difficile is a nosocomial, Gram-positive, strictly anaerobic, spore-forming pathogen capable of colonizing and proliferating in the human intestine. In bacteria, it has been shown that the Toxin-Antitoxin systems mediate the cellular response to external stress by initiating processes such as biofilm formation and programmed cell death. This work aims to evaluate the functionality of four type II TA modules of Clostridioides difficile R20291. We performed bioinformatic analysis to search for putative TA systems using the TADB platform. Then we performed a heterologous expression assay to evaluate the functionality of these systems. Our results showed that the MazEF and RelBE systems were functional, suggesting that their corresponding toxins possess an endoribonuclease activity. In conclusion, MazEF and RelBE systems of C. difficile R20291 are functional in a heterologous expression system.
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